Bilirubin and the polarographic Brdicka reaction.

The bilirubin-albumin solution containing 16 ing/100 ml of bilirubin and 2.5 g/100 ml of albumin was made according to Shinowara (i). The blank solution without bilirubin contained the same amount of albumin as the test solution. The Brdicka ilonfiltrate reaction was carried out as follows (2): 0.1 nil of test/blailk solution was added to 1.4 ml of 0.9% (w/v) NaC1 solution, and 1.5 ml of 0.25 N NaOH solution was added. The mixture was placed in a water bath (18-20#{176}) and a sample of 0.1 ml from tile mixture was pipetted to 4.0 ml of Brdicka HC0+3 solution, and polarographed between -0.8 to -1.7 using silver wire as anode. The samples for polarography were taken immediately, and then 10, 20, 30, and 45 mm following the addition of NaOH. The height of the second peak of protein double wave was measured. The results which are means of two series are presented in Fig 1.